Review




Structured Review

STEMCELL Technologies Inc roboseptm buffer
Roboseptm Buffer, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/roboseptm+buffer/robosep+buffer/pm40277913-72-6-8
Average 90 stars, based on 1 article reviews
roboseptm buffer - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Red Blood Cell Lysis:

Article Title: Optical Genome Mapping Reveals the Complex Genetic Landscape of Myeloma.
Article Snippet: Plasma cells were then purified using EasySepTM Human CD138 Positive Selection Kit II (#17887A, Stemcell technologies, Vancouver, BC, Canada) according to the manufacturer’s procedures. .. Briefly, pelleted BMA samples were washed with RoboSepTM Buffer (#20104, Stemcell technologies) and red blood cells were lysed with 1× EasySepTM Red Blood Cell Lysis Buffer (#20110, Stemcell technologies). .. Samples were then subject to CD138-positive selection using the semi-automatic Robosep-STM instrument (STEMCELL Technologies, Vancouver, BC, Canada) using immunomagnetic microbeads coated with anti-CD138 monoclonal antibody.

Control:

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. PE-CyTM 7 Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 557646) FITC Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 556649) V450 Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 561504) Alexa Fluor ® 700 Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 557882) APC-H7 Mouse IgG 1 , k Isotype Control (BD Biosciences, catalog number: 561427) PE Mouse IgG 2a , κ Isotype Control (BD Biosciences, catalog number: 558595) Dye-eFluor 506 (Invitrogen, catalog number: 65–0866-14) DPBS (1×), Dulbecco’s Phosphate-Buffered Saline (Corning Cellgro, catalog number: 21–031-CV), store at 4 °C for use after opening bottle Dry Ice HBSS (1×), Hanks Balanced Salt Solution (Thermo Fischer Scientific, GibcoTM, catalog number: 141775–095), store at 4 °C for use after opening bottle IMDM (1×) (Thermo Fischer Scientific, GibcoTM, catalog number: 12440–053), store at 4 °C for use after opening bottle Lymphocyte separation medium (Corning, catalog number: 25–072-CV), store at 4 °C for use after opening bottle Penicillin/Streptomycin (Thermo Fischer Scientific, GibcoTM, catalog number: 15140–122), store at −18 °C freezer before opening bottle and then store at 4 °C for subsequent use RPMI 1640 (1×) with L-glutamine and 25 mM HEPES (Corning Cellgro, catalog number: 10–041-CV) 0.05% Trypsin EDTA (1×) (Thermo Fischer Scientific, GibcoTM, catalog number: 25300–120), store at −20 °C freezer before opening bottle and then store at 4 °C for subsequent use RoboSepTM Buffer (Stemcell Technologies, catalog number: 20104) Formalin 10% RPMI (see Recipes ) 10% IMDM (see Recipes ) .. Tissue culture flask vented caps, 70 ml (Falcon, catalog number: 353109) Tissue culture flask vented caps, 250 ml (Falcon, catalog number: 353110) Pipettes Rocker Incubator (Thermo Electron Corporation, NAPCO SERIES 8000 WJ, CO 2 Incubator) Centrifuge: Sorvall Legend RT+ Centrifuge (Thermo Scientific) Freezer Luminometer (Promega GloMax Navigator, GM2000) LN2 freezer Magnets (Stem Cell Technologies, catalog number: 96290) Light Microscope 4 °C refrigerator Flow cytometer (BD, model: LSR II) Water Bath (Fisher Scientific, model: 2223) Haemocytometer 0.100 mm deep (Hausser Scientific)

Saline:

Article Title: TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4 + T Cells
Article Snippet: .. PE-CyTM 7 Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 557646) FITC Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 556649) V450 Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 561504) Alexa Fluor ® 700 Mouse IgG 1 , κ Isotype Control (BD Biosciences, catalog number: 557882) APC-H7 Mouse IgG 1 , k Isotype Control (BD Biosciences, catalog number: 561427) PE Mouse IgG 2a , κ Isotype Control (BD Biosciences, catalog number: 558595) Dye-eFluor 506 (Invitrogen, catalog number: 65–0866-14) DPBS (1×), Dulbecco’s Phosphate-Buffered Saline (Corning Cellgro, catalog number: 21–031-CV), store at 4 °C for use after opening bottle Dry Ice HBSS (1×), Hanks Balanced Salt Solution (Thermo Fischer Scientific, GibcoTM, catalog number: 141775–095), store at 4 °C for use after opening bottle IMDM (1×) (Thermo Fischer Scientific, GibcoTM, catalog number: 12440–053), store at 4 °C for use after opening bottle Lymphocyte separation medium (Corning, catalog number: 25–072-CV), store at 4 °C for use after opening bottle Penicillin/Streptomycin (Thermo Fischer Scientific, GibcoTM, catalog number: 15140–122), store at −18 °C freezer before opening bottle and then store at 4 °C for subsequent use RPMI 1640 (1×) with L-glutamine and 25 mM HEPES (Corning Cellgro, catalog number: 10–041-CV) 0.05% Trypsin EDTA (1×) (Thermo Fischer Scientific, GibcoTM, catalog number: 25300–120), store at −20 °C freezer before opening bottle and then store at 4 °C for subsequent use RoboSepTM Buffer (Stemcell Technologies, catalog number: 20104) Formalin 10% RPMI (see Recipes ) 10% IMDM (see Recipes ) .. Tissue culture flask vented caps, 70 ml (Falcon, catalog number: 353109) Tissue culture flask vented caps, 250 ml (Falcon, catalog number: 353110) Pipettes Rocker Incubator (Thermo Electron Corporation, NAPCO SERIES 8000 WJ, CO 2 Incubator) Centrifuge: Sorvall Legend RT+ Centrifuge (Thermo Scientific) Freezer Luminometer (Promega GloMax Navigator, GM2000) LN2 freezer Magnets (Stem Cell Technologies, catalog number: 96290) Light Microscope 4 °C refrigerator Flow cytometer (BD, model: LSR II) Water Bath (Fisher Scientific, model: 2223) Haemocytometer 0.100 mm deep (Hausser Scientific)

Selection:

Article Title: The Antitumor Effect of Xihuang Pill on Treg Cells Decreased in Tumor Microenvironment of 4T1 Breast Tumor-Bearing Mice by PI3K/AKT~AP-1 Signaling Pathway
Article Snippet: After washing with permeabilization buffer, the cells were analyzed by flow cytometry FACSCalibur (BD, USA). .. The concentrations of the cells were adjusted to 1 × 10 8 mL −1 with the RoboSepTM buffer (STEMCELL, Canada) and the next steps were both in accordance with the manufacturer's instructions of EasySepTM Mouse CD25 Regulatory T Cell Positive Selection Kit (STEMCELL, Canada). ..

Article Title: Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy.
Article Snippet: .. The cells were resuspended in RoboSepTM buffer (Stemcell, #20104) and incubated according to the EasySepTM human CD34 positive selection kit II (Stemcell, #17856) technical manual for CD34 positive cell sorting and purification. ..

Centrifugation:

Article Title: Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction.
Article Snippet: Blood was incubated on ice for 15 min with ammonium chloride solution (STEMCELL Technologies, 07800, Canada). .. After centrifugation, the supernatant was discarded and the cells were washed with RoboSepTM Buffer (STEMCELL Technologies, 20104, Canada) and resuspended. .. Subsequently, the sample was transferred to a polystyrene round-bottom tube with 25 μL normal rat serum (Abcam, ab13551, USA) and 25 μL selection cocktail (STEMCELL Technologies, 14766, Canada) in each well, and the sample was incubated at room temperature for 5 min. RapidSpheresTM (40 μL/1 mL) and RoboSepTM buffer were added to the sample and the tube was placed in an EasySepTM magnet (STEMCELL Technologies, 18000, Canada) at room temperature for 3 min. After removing the supernatant from the tube in the magnet, the cells were resuspended in sterile PBS.

Incubation:

Article Title: Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy.
Article Snippet: .. The cells were resuspended in RoboSepTM buffer (Stemcell, #20104) and incubated according to the EasySepTM human CD34 positive selection kit II (Stemcell, #17856) technical manual for CD34 positive cell sorting and purification. ..

FACS:

Article Title: Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy.
Article Snippet: .. The cells were resuspended in RoboSepTM buffer (Stemcell, #20104) and incubated according to the EasySepTM human CD34 positive selection kit II (Stemcell, #17856) technical manual for CD34 positive cell sorting and purification. ..

Purification:

Article Title: Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy.
Article Snippet: .. The cells were resuspended in RoboSepTM buffer (Stemcell, #20104) and incubated according to the EasySepTM human CD34 positive selection kit II (Stemcell, #17856) technical manual for CD34 positive cell sorting and purification. ..

In Vitro:

Article Title: Brain cancer induces systemic immunosuppression through release of non-steroid soluble mediators.
Article Snippet: Data were analysed on FlowJo 10.1 (FlowJo LLC, Ashland, OR). .. CFSE labelling and in vitro T-cell proliferation assay Isolated cells from spleens and lymph nodes of naı̈ve unmanipulated C57BL/6 mice were placed in RoboSepTM buffer (Stemcell Technologies) at 5 107 cells/ml. .. CFSE (1 ml/ml) [BioLegend, 5 mM in dimethyl sulphoxide (DMSO) stock] was added at a final concentration of 5 mM followed by a 10-min incubation at room temperature.

Proliferation Assay:

Article Title: Brain cancer induces systemic immunosuppression through release of non-steroid soluble mediators.
Article Snippet: Data were analysed on FlowJo 10.1 (FlowJo LLC, Ashland, OR). .. CFSE labelling and in vitro T-cell proliferation assay Isolated cells from spleens and lymph nodes of naı̈ve unmanipulated C57BL/6 mice were placed in RoboSepTM buffer (Stemcell Technologies) at 5 107 cells/ml. .. CFSE (1 ml/ml) [BioLegend, 5 mM in dimethyl sulphoxide (DMSO) stock] was added at a final concentration of 5 mM followed by a 10-min incubation at room temperature.

Isolation:

Article Title: Brain cancer induces systemic immunosuppression through release of non-steroid soluble mediators.
Article Snippet: Data were analysed on FlowJo 10.1 (FlowJo LLC, Ashland, OR). .. CFSE labelling and in vitro T-cell proliferation assay Isolated cells from spleens and lymph nodes of naı̈ve unmanipulated C57BL/6 mice were placed in RoboSepTM buffer (Stemcell Technologies) at 5 107 cells/ml. .. CFSE (1 ml/ml) [BioLegend, 5 mM in dimethyl sulphoxide (DMSO) stock] was added at a final concentration of 5 mM followed by a 10-min incubation at room temperature.

Article Title: Biological effects of melatonin on osteoblast/osteoclast cocultures, bone, and quality of life: Implications of a role for MT2 melatonin receptors, MEK1/2, and MEK5 in melatonin-mediated osteoblastogenesis.
Article Snippet: Freshly collected blood was mixed with Ficoll- PaqueTM Plus (Amersham Pharmacia Biotech, Sweden) and centrifuged twice (400 g for 30- 40 minutes at 18- 20°C without break). .. Human PBMCs were isolated from centrifuged blood and resuspended in RoboSepTM buffer (StemCell Technologies, USA). .. Pure human PBMCs were separated magnetically from the cell suspension using EasySepTM negative selection human monocyte enrichment kit without CD16 depletion (StemCell Technologies, Cambridge, MA) and purple EasySepTM magnet (StemCell Technologies, Cambridge, MA) per manufacturer’s instructions.



Similar Products

90
STEMCELL Technologies Inc roboseptm buffer
Roboseptm Buffer, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/roboseptm+buffer/robosep+buffer/pm40277913-72-6-8
Average 90 stars, based on 1 article reviews
roboseptm buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Merck KGaA 0.5 ml roboseptm buffer
0.5 Ml Roboseptm Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/roboseptm+buffer/0+5+ml+roboseptm+buffer/pmc08772903-102-9-32
Average 90 stars, based on 1 article reviews
0.5 ml roboseptm buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results